Control of Expression Random Retrieval

In a PCR reaction, the DNA is heated at the start of the cycle to around 95 degrees C. What is the reason for this?

Sometimes, two marker genes are used: The first marker gene is often an antibiotic resistance gene to show if the plasmid is present; the bacteria can only grow on a plate containing the antibiotic, if they have the antibiotic resistance gene on the plasmid. The second is often a gene with a colour change, which is broken by the insertion of the fragment.

e.g. An example of a gene to test for the presence of a fragment is Green Fluorescent Protein (GFP) glowing or not. What would a bacterial plate look like that contained an antibiotic, and was spread with bacteria which had been transformed with a plasmid and had a new gene fragment in?

We cannot see the proteins made by recombinant plasmids, nor the plasmids themselves. How do we know the bacteria have been transformed properly and contain a recombinant plasmid ( = plasmid and new gene fragment) ?

Which enzyme do we use to convert mRNA into cDNA?

Can extra methylation be removed from the DNA?

What are iPS cells?

Which molecule does the SIRNA / protein complex (RISC) bind to?

What forms the substrate in a Transcription complex formed from OOR and RNA Polymerase?

Where is the oestrogen receptor located?

The mRNA travels to the ribosome, where it feeds between the two subunits, exposing the template bases. tRNA molecules each carry a specific ______6____at the top, with a complementary sequence of three bases called an _____7_____ at the bottom. The tRNA anticodons then complementary base pair with the mRNA ____8____.

In the ribosome there are 3 binding sites for tRNA, two where a ____9____ bond is formed between the carried amino acids. These amino acids then form a ____10_____ chain which leaves the ribosome.

The third site is a departure site, where the tRNA which has released its amino acid departs to pick up a new amino acid. Once the entire mRNA sequence has been ____11____, it can either pass into another ribosome to make another polypeptide or be degraded.

What is the removal of introns called?

Fill in the missing word : Causes of Mutations A mutation is any change to the order of __12___ in the genetic code. These occur naturally as the enzyme _____13_____ replicates the DNA and makes mistakes. The incidence can also be produced due to exposure to certain agents called mutagenic agents. These can be chemicals such as nitrous acid, which converts cytosine to uracil.

Nitrous acid would therefore cause a __14___ mutation. Ethidium Bromide is used to stain DNA during gel electrophoresis. It can insert between bases, acting in a similar way to an addition mutation and therefore causing a __15___ along the rest of the gene. UV radiation is also a mutagenic agent.

It can cause bonds to form between pyrimidine bases ( thymine and cytosine). This bonding causes polymerases to misread or stop working all together at that point.

What word describes when a mutation causes all the following triplet codes to be misread in a different frame?

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop Here is the original sequence : TTCTTGTTATCATAA. Here is a mutated sequence: TTCTTGTTATAACATAA.

What type of mutation has occurred?

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop Here is the original sequence : TTCTTGTTATCATAA. Here is a mutated sequence: TTCTTATTATCATAA.

What type of mutation has occurred ?

What is the position of gene on a chromosome called?

If a section of DNA contains 21% base C, what percentage will base A have?

Which two molecules does DNA Polymerase join together?

Name the type of bond which forms the backbone of the DNA strand.

Which component of a nucleotide contains nitrogen atoms?