Control of Expression Random Retrieval

We cannot see the proteins made by recombinant plasmids, nor the plasmids themselves. How do we know the bacteria have been transformed properly and contain a recombinant plasmid ( = plasmid and new gene fragment) ?

Using bacteria is an example of what type of DNA Amplification?

Which bond to restriction endonucleases ( restriction enzymes) break?

How would the cDNA base sequence of a gene differ to the normal genomic base sequence of a gene?

Can extra methylation be removed from the DNA?

What effect will having a high methylation of a promoter sequence , have on expression of that gene?

What is 'Epigenetics'?

What are iPS cells?

Cardiomyocytes are an example of what type of stem cell?

Which type of stem cells are not found in adults?

After fertilisation, zygotes ( fertilised eggs) divide by which process?

Which molecule does the SIRNA / protein complex (RISC) bind to?

What do we call a transcription factor that 'turns on ' genes?

The mRNA travels to the ribosome, where it feeds between the two subunits, exposing the template bases. tRNA molecules each carry a specific amino acid at the top, with a complementary sequence of three bases called an anti-codon at the bottom. The tRNA anticodons then complementary base pair with the mRNA ____8____.

In the ribosome there are 3 binding sites for tRNA, two where a ____9____ bond is formed between the carried amino acids. These amino acids then form a ____10_____ chain which leaves the ribosome. The third site is a departure site, where the tRNA which has released its amino acid departs to pick up a new amino acid.

Once the entire mRNA sequence has been ____11____, it can either pass into another ribosome to make another polypeptide or be degraded.

Which of the following would NOT increase your exposure to oestrogen?

Methylation of DNA is when a methyl group ( CH3) is added onto CpG bases ( cytosine and guanine next to each other). This can stop DNA polymerase from binding.

What effect will increased methylation (Hypermethylation) have on transcription?

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop What would be the order of amino acids in a polypeptide coded from this sequence?

TTCTTGTTATCATAA

Which direction can DNA polymerase only extend the new strand of DNA?

Which two molecules does DNA Polymerase join together?

Which of the following is NOT a difference between RNA and DNA?