Control of Expression Random Retrieval

What do we call it if the restriction enzyme cuts both strands of DNA in a staggered position to create an overhang?

How would the cDNA base sequence of a gene differ to the normal genomic base sequence of a gene?

Which enzyme do we use to convert mRNA into cDNA?

Sometimes when trying to find a gene which important in a particular disease, it is useful to find out what genes the diseased cells have used (expressed) by extracting mRNA rather than DNA. However, mRNA cannot be used in genetic modifications. What do we need to change it into to be able to use it?

Can extra methylation be removed from the DNA?

Which area of DNA is particularly affected by methylation?

Which molecule does the SIRNA / protein complex (RISC) bind to?

What does the 'si' stand for in siRNA?

Which level of protein structure will the binding of oestrogen alter?

What is the oestrogen receptor called when the oestrogen has bound?

Where is the oestrogen receptor located?

What type of hormone is oestrogen?

What do we call a transcription factor that 'turns off ' genes?

Fill in the missing word : Causes of Mutations A mutation is any change to the order of __12___ in the genetic code. These occur naturally as the enzyme _____13_____ replicates the DNA and makes mistakes.

The incidence can also be produced due to exposure to certain agents called mutagenic agents. These can be chemicals such as nitrous acid, which converts cytosine to uracil. Nitrous acid would therefore cause a __14___ mutation. Ethidium Bromide is used to stain DNA during gel electrophoresis. It can insert between bases, acting in a similar way to an addition mutation and therefore causing a __15___ along the rest of the gene.

UV radiation is also a mutagenic agent. It can cause bonds to form between pyrimidine bases ( thymine and cytosine). This bonding causes polymerases to misread or stop working all together at that point.

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop Here is the original sequence : TTCTTGTTATCATAA. Here is a mutated sequence: TTCTTATTATCATAA.

Will this mutation affect the primary structure of the polypeptide ?

What does the term 'Degenerate' mean when referring to the genetic code?

What is the position of gene on a chromosome called?

Which word describes the sequence of three bases on DNA that code for an amino acid?

Which word does NOT describe the genetic code?

Name the enzyme that breaks the hydrogen bonds between complimentary bases during DNA replication.