Control of Expression Random Retrieval

We cannot see the proteins made by recombinant plasmids, nor the plasmids themselves. How do we know the bacteria have been transformed properly and contain a recombinant plasmid ( = plasmid and new gene fragment) ?

If a bacterium contains a recombinant plasmid, then it will express the new 'foreign' gene as well as it's own. Which of the following is not a product made by recombinant bacteria?

What do we call enzymes that cut DNA at specific base sequences?

Which enzyme do we use to convert mRNA into cDNA?

What effect will having a high methylation of a promoter sequence , have on expression of that gene?

What word describes the inner cell mass now?

What effect will siRNA have on expression of a gene?

Where is the oestrogen receptor located?

What do we call a transcription factor that 'turns off ' genes?

What do we call a transcription factor that 'turns on ' genes?

Where are transcription factors often stored?

What are the proteins called that also need to bind to activate RNA polymerase?

Which of the following describes the structure of DNA?

If hypermethylation happens to a proto-oncogene, what effect will this have on the rate of mitosis?

Fill in the missing word : Causes of Mutations A mutation is any change to the order of __12___ in the genetic code. These occur naturally as the enzyme _____13_____ replicates the DNA and makes mistakes.

The incidence can also be produced due to exposure to certain agents called mutagenic agents. These can be chemicals such as nitrous acid, which converts cytosine to uracil. Nitrous acid would therefore cause a __14___ mutation. Ethidium Bromide is used to stain DNA during gel electrophoresis. It can insert between bases, acting in a similar way to an addition mutation and therefore causing a __15___ along the rest of the gene.

UV radiation is also a mutagenic agent. It can cause bonds to form between pyrimidine bases ( thymine and cytosine). This bonding causes polymerases to misread or stop working all together at that point.

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop Here is the original sequence : TTCTTGTTATCATAA. Here is a mutated sequence: TTCTTATTATCATAA.

Will this mutation affect the primary structure of the polypeptide ?

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop What would be the order of amino acids in a polypeptide coded from this sequence?

TTCTTGTTATCATAA

Which word describes the sequence of three bases on DNA that code for an amino acid?

Which type of DNA is not circular?

Each complementary strand of DNA runs in opposite directions. What word describes this?