Control of Expression Random Retrieval

PCR allows DNA to replicate by semi-conservative replication. But what word best describes the rate at which it amplifies?

Sometimes, two marker genes are used: The first marker gene is often an antibiotic resistance gene to show if the plasmid is present; the bacteria can only grow on a plate containing the antibiotic, if they have the antibiotic resistance gene on the plasmid. The second is often a gene with a colour change, which is broken by the insertion of the fragment.

e.g. An example of a gene to test for the presence of a fragment is Green Fluorescent Protein (GFP) glowing or not. What would a bacterial plate look like that contained an antibiotic, and was spread with bacteria which had been transformed with a plasmid and had a new gene fragment in?

What is the active site of a restriction endonuclease complementary too?

The morula hollows out into a hollow sphere, with a small group of cells at the bottom called teh inner cell mass which go on to develop into an embryo. What do the outer cells go on to make?

Which term is used to describe the full process of genetic code in DNA being turned into a polypeptide chain?

What forms the substrate in a Transcription complex formed from OOR and RNA Polymerase?

Which level of protein structure will the binding of oestrogen alter?

What do we call a transcription factor that 'turns off ' genes?

What do we call a transcription factor that 'turns on ' genes?

The mRNA travels to the ribosome, where it feeds between the two subunits, exposing the template bases. tRNA molecules each carry a specific amino acid at the top, with a complementary sequence of three bases called an _____7_____ at the bottom. The tRNA anticodons then complementary base pair with the mRNA ____8____.

In the ribosome there are 3 binding sites for tRNA, two where a ____9____ bond is formed between the carried amino acids. These amino acids then form a ____10_____ chain which leaves the ribosome. The third site is a departure site, where the tRNA which has released its amino acid departs to pick up a new amino acid.

Once the entire mRNA sequence has been ____11____, it can either pass into another ribosome to make another polypeptide or be degraded.

The mRNA travels to the ribosome, where it feeds between the two subunits, exposing the template bases. tRNA molecules each carry a specific ______6____at the top, with a complementary sequence of three bases called an _____7_____ at the bottom. The tRNA anticodons then complementary base pair with the mRNA ____8____.

In the ribosome there are 3 binding sites for tRNA, two where a ____9____ bond is formed between the carried amino acids. These amino acids then form a ____10_____ chain which leaves the ribosome.

The third site is a departure site, where the tRNA which has released its amino acid departs to pick up a new amino acid. Once the entire mRNA sequence has been ____11____, it can either pass into another ribosome to make another polypeptide or be degraded.

If hypomethylation ( reduced methylation) happens to a tumour suppressor gene what effect will this have on the rate of mitosis?

If hypermethylation happens to a tumour suppressor gene, what effect will this have on the rate of mitosis?

Fill in the missing word : Causes of Mutations A mutation is any change to the order of __12___ in the genetic code. These occur naturally as the enzyme _____13_____ replicates the DNA and makes mistakes. The incidence can also be produced due to exposure to certain agents called mutagenic agents.

These can be chemicals such as nitrous acid, which converts cytosine to uracil. Nitrous acid would therefore cause a __14___ mutation. Ethidium Bromide is used to stain DNA during gel electrophoresis. It can insert between bases, acting in a similar way to an addition mutation and therefore causing a __15___ along the rest of the gene. UV radiation is also a mutagenic agent.

It can cause bonds to form between pyrimidine bases ( thymine and cytosine). This bonding causes polymerases to misread or stop working all together at that point.

What type of mutation causes a sequence of bases to be reversed?

Which of the following is a difference between prokaryotic and eukaryotic DNA?

Which direction can DNA polymerase only extend the new strand of DNA?

The deoxyribose sugar at the end of a DNA molecule can either have a bond available to join with carbon number 3 ( 3' or 3 prime) or carbon number 5 (5' or 5 prime). DNA polymerase can only join to one end, which end can it bind to and then extend?

Which of the following is NOT a difference between RNA and DNA?

Name the components of a DNA nucleotide.