U1T8 – DNA Replication

Which enzyme breaks the bonds between complementary base pairs in preparation for DNA replication?

What type of bonds are broken between the DNA bases when replication starts?

Which molecules bind to the exposed bases when DNA becomes single stranded?

Which enzyme works along the template DNA strand, joining new monomers together?

How many bonds form between the Bases C and G when the base pair?

A single DNA strand has two ends - one called 5 prime (5') and one called 3 prime (3'). To which end of the new DNA strand does the enzyme bind in DNA replication?

Which direction does the enzyme move along the template strand A?

In the diagram, what name is given to strand A?

True or False : The enzyme DNA polymerase can only move in one direction along the template strand.

True or False : The enzyme DNA polymerase can only bind to double stranded DNA?

What name is given to the DNA fragments labelled C in the diagram?

Which two letters represent 5' ends of the DNA strand?

The diagram shows a template strand of DNA. Which of the following is the order the first 5 new bases will be added (first in the sequence, would be first to be added)?

Which pair of scientists proved the method by which DNA replicated?

By which method does DNA replicate?

In this experiment bacteria were grown in a growth medium containing N¹⁵. What isN¹⁵?

In this experiment, bacteria were grown for many generations in a medium containing N¹⁵. The bacteria were then removed from the N¹⁵ , and placed in medium containing .N^14.. When The DNA from this first generation was centrifuged ( tube A), what pattern did the DNA bands form?

In this experiment bacteria grown in N¹⁵, were then treansfered to N¹⁴, and allowed to divide for two generations ( tubeB). Describe what the cenrifuged DNA bands would look like.

If DNA replicated by a Conservative method, what would the DNA bands look like after one generation in N¹⁴ medium(Tube A)?

If DNA replicated by a Dispersive method ( fragments from each strand mixed), what would the DNA bands look like after two generations in N¹⁴ medium (Tube B)?