Control of Expression Random Retrieval

This quiz contains all the questions in the Control of Expression section. The website will pick 20 questions at random.

1 / 20

Why do we need two primers in PCR?

Incase one breaks

One for each end of the same strand.

One to attach to the start of one strand (sense strand) , and the other primer to attach to the opposite end of the other strand (anti-sense strand).

For use in alternate cycles.

2 / 20

What enzyme is needed in a PCR reaction ?

DNA polymerase

RNA polymerase

DNA Ligase

DNA Helicase

3 / 20

If a bacterium contains a recombinant plasmid, then it will express the new 'foreign' gene as well as it's own. Which of the following is not a product made by recombinant bacteria?

Human Insulin for Diabetics

Clotting Factors for Haemophiliacs

Enzymes for use in Cheese making

Glycogen for use by atheletes before endurance events.

4 / 20

What bond does DNA ligase create?

Hydrogen

Glycosidic

Phosphodiester

Peptide

5 / 20

How would the cDNA base sequence of a gene differ to the normal genomic base sequence of a gene?

No exons

No Introns

Double stranded

Single stranded

6 / 20

Which enzyme do we use to convert mRNA into cDNA?

Reverse Translate

Reverse Transcribase

Reverse Transcriptase

Reverse mRNAase

7 / 20

What effect will increased acetylation have on a gene?

Increase expression

Decrease expression

Stay at a steady level

Stay at whatever it was before.

8 / 20

Cell differentiation is the process of stem cells becoming more specialised into different types of cell. e.g. Muscle, bone etc. But what is the cause of this differentiation?

All genes are expressed (turned 'on'), but in a particular order.

All genes are expressed (turned 'on'), but some are turned off again later.

Some genes are expressed (turned 'on'), others are not.

Some genes are expressed, but in a random order.

9 / 20

In humans, the zygote ( fertilised egg ) divides into a ball of cells called a Morula. What word describes the cells contained in a morula?

Totipotent

Pluipotent

Multipotent

Unipotent

10 / 20

Which molecule does the SIRNA / protein complex (RISC) bind to?

Double stranded DNA

Single Stranded DNA

tRNA

mRNA

11 / 20

What does the 'si' stand for in siRNA?

Small In-Vitro

Small In-Vivo

Small Irrational

Small Interfering

12 / 20

Where is the oestrogen receptor located?

Nucleus

Cytoplasm

Endoplasmic Reticulum

Golgi Body

13 / 20

What word describes when a mutation causes all the following triplet codes to be misread in a different frame?

Non-disjunction

Frameshift

Reading Shift

Triplet Misread.

14 / 20

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop Here is the original sequence : TTCTTGTTATCATAA. Here is a mutated sequence: TTCTTGTTATAACATAA.

What type of mutation has occurred?

Substitution

Addition

Deletion

Inversion

15 / 20

TTA = Leu TTG = Leu TTC = Phe TCA = Ser TAA = Stop What would be the order of amino acids in a polypeptide coded from this sequence?

TTCTTGTTATCATAA

Phe - leu - Leu - Ser - Ser

Phe - Ser - Leu - Leu - STOP

Phe - Leu- Leu - Ser - STOP

16 / 20

What is the position of gene on a chromosome called?

Locus

Allele

Centromere

Centriole

17 / 20

Which word does NOT describe the genetic code?

Degenerate

Universal

Non-Overlapping

Interrupted

18 / 20

Which of the following is a difference between prokaryotic and eukaryotic DNA?

Contains 4 bases : A,T, C and G

Uses DNA polymerase to replicate

DNA is wrapped around histone proteins

H bonds hold the complementary base pairs together

19 / 20

What does the C stand for in the genetic code?

Cytosine

Cytotine

Cytonine

Cytonide

20 / 20

What does the A stand for in the genetic code?

Adenosine

Adenotine

Adenine

Adenide

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